
101
20
-20
-15
-10
-5
0
5
10
15
200180160140120100
SYSTOLIC PRESSURE
Mean difference(MD): 0 mmHg
Standard deviation(SD): 6 mmHg
mean + 2 SD
mean
mean - 2 SD
Average of test and reference methods (mmHg)
Difference between test and reference methods (mmHg)
(EXAMPLE) Agreement between test and reference methods for systolic pressure. Hypothetical data
SpO
2
Processing
Pulse oximetry works by applying a sensor to a pulsating arteriolar vascular bed. The
sensor contains a dual light source and photodetector. Bone, tissue, pigmentation, and
venous vessels normally absorb a constant amount of light over time. The arteriolar bed
normally pulsates and absorbs variable amounts of light during systole and diastole, as
blood volume increases and decreases. The ratio of light absorbed at systole and diastole
is translated into an oxygen saturation measurement (SpO
2
). Because a measurement of
SpO
2
is dependent upon light from the sensor, excessive ambient light can interfere with
this measurement.
Pulse oximetry is based on two principles: that oxyhemoglobin and deoxyhemoglobin
differ in their absorption of red and infrared light (spectrophotometry), and that the volume
of arterial blood in tissue (and hence, light absorption by that blood) changes during the
pulse (plethysmography). The monitor determines SpO
2
by passing red and infrared light
into an arteriolar bed and measuring changes in light absorption during the pulsatile cycle.
Red and infrared low-voltage light-emitting diodes (LED) in the oximetry SpO
2
sensor
serve as light sources; a photo diode serves as the photo detector. Because
oxyhemoglobin and deoxyhemoglobin differ in light absorption, the amount of red and
infrared light absorbed by blood is related to hemoglobin oxygen saturation. To identify
the oxygen saturation of arterial hemoglobin, the monitor uses the pulsatile nature of
arterial flow. During systole, a new pulse of arterial blood enters the vascular bed, and
blood volume and light absorption increase. During diastole, blood volume and light
absorption reach their lowest point. The monitor bases its SpO
2
measurements on the
difference between maximum and minimum absorption (measurements at systole and
diastole). By doing so, it focuses on light absorption by pulsatile arterial blood, eliminating
the effects of nonpulsatile absorbers such as tissue, bone, and venous blood.
Automatic Calibration (Nellcor module)
Because light absorption by hemoglobin is wavelength dependent and because the mean
wavelength of LEDs varies, an oximeter must know the mean wavelength of the SpO
2
sensor’s red LED to accurately measure SpO
2
. During monitoring, the instrument’s
software selects coefficients that are appropriate for the wavelength of that individual
sensor’s red LED; these coefficients are then used to determine SpO
2
.
Additionally, to compensate for differences in tissue thickness, the light intensity of the
sensor’s LEDs is adjusted automatically.
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